Coding

Part:BBa_K179003

Designed by: Koji Tanaka   Group: iGEM09_Todai-Tokyo   (2009-10-18)

Low density lipoprotein receptor

LDLR (Low-Density Lipoprotein Receptor) from a human cDNA library. The protein localizes to the plasma membrane where it binds to and mediates transport of LDL into the cell by clathrin-mediated endocytosis. LDL is a lipoprotein that transports macromolecules such as cholesterol and triglycerides from the liver to peripheral tissues via the blood stream. High blood LDL levels may cause serious diseases such as arteriosclerosis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 718
    Illegal BglII site found at 1704
    Illegal BamHI site found at 1077
    Illegal BamHI site found at 1448
    Illegal BamHI site found at 1572
    Illegal XhoI site found at 2544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
    Illegal NgoMIV site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None